![]() If a glass tube is used, the tube can be “scored” above the buffy coat, using a diamond pen or the side of a microscope slide, i.e. ![]() However, these are not always available and glass microhematocrit tubes can be used. SAFEPETTE ® dispenser), which transfers the plasma to the refractometer should be used. SAFE-CAP ® plain capillary tubes) and a plasma-expeller (e.g. Ideally plastic microhematocrit tubes (which cannot be broken, e.g. ![]() The tube should consist of packed red blood cells (at this stage, a packed cell volume or PCV can be measured), the buffy coat (platelets and WBC) and overlying plasma (the color/nature of the supernatant plasma can be visually assessed – see below) (see image to right). The tube is then sealed, placed in a microhematocrit centrifuge, centrifuged for 3 minutes (or longer) and then removed from the centrifuge. To perform this test, a microhematocrit tube is filled with EDTA-anticoagulated blood using capillary action.
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